CELL CULTURE
Human SerumCell Culture > Human Serum
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| Human AB Serum | |
| HP1022 | Human AB Serum Spec Sheet |
| Human AB plasma is collected from healthy donors at FDA-licensed facilities located entirely within the U.S. These individual units are converted to serum and then pooled, filtered, bottled and cell culture tested for mycoplasma, endotoxin, and sterility. Each individual unit is tested and found negative for all required viral markers via FDA-Approved methods (HBsAg, HIV-1, HIV-2, HCV, HIV-1Ag or HIV-1 NAT, ALT and syphilis). | |
| HP1022HI | Human AB Serum Heat-InactivatedSpec Sheet |
| The Valley Biomedical heat inactivation protocol is to bring the temperature of the material to 56 deg C and maintain this for 30 minutes. Some precipitation may be evident, which can be removed, if desired, by filtration. | |
| Human AB Serum (off-the-clot) | |
| HS1017 | Human AB Serum (off-the-clot)Spec Sheet |
| Human AB serum is collected from the whole blood donation of healthy male donors at FDA-licensed facilities located entirely within the U.S. The whole blood is allowed to spontaneously clot before the serum component is separated. These individual units of serum are then pooled, filtered, bottled and cell culture tested for mycoplasma, endotoxin, and sterility. Each individual unit is tested and found negative for all required viral markers via FDA-Approved methods (HBsAg, HIV-1, HIV-2, HCV, HIV-1Ag or HIV-1 NAT, ALT and syphilis). | |
| HS1017HI | Human AB Serum (off-the-clot) Heat-InactivatedSpec Sheet |
| The Valley Biomedical heat inactivation protocol is to bring the temperature of the material to 56 deg C and maintain this for 30 minutes. Some precipitation may be evident, which can be removed, if desired, by filtration. | |
| Human Serum | |
| HS1021 | Human SerumSpec Sheet |
| Human plasma is collected from healthy donors at FDA-licensed facilities located entirely within the U.S. These individual units are converted to serum and then pooled, filtered, bottled and cell culture tested for mycoplasma, endotoxin, and sterility. Each individual unit is tested and found negative for all required viral markers via FDA-Approved methods (HBsAg, HIV-1, HIV-2, HCV, HIV-1Ag or HIV-1 NAT, ALT and syphilis). | |
| HS1021HI | Human Serum Heat-InactivatedSpec Sheet |
| The Valley Biomedical heat inactivation protocol is to bring the temperature of the material to 56 deg C and maintain this for 30 minutes. Some precipitation may be evident, which can be removed, if desired, by filtration. | |
| Human Serum (off-the-clot) | |
| HS1004C | Human Serum (off-the-clot)Spec Sheet |
| Human serum is collected from the whole blood donation of healthy male donors at FDA-licensed facilities located entirely within the U.S. The whole blood is allowed to spontaneously clot before the serum component is separated. These individual units of serum are then pooled, filtered, bottled and cell culture tested for mycoplasma, endotoxin, and sterility. Each individual unit is tested and found negative for all required viral markers via FDA-Approved methods (HBsAg, HIV-1, HIV-2, HCV, HIV-1Ag or HIV-1 NAT, ALT and syphilis). | |
| HS1004CHI | Human Serum (off-the-clot) Heat-InactivatedSpec Sheet |
| The Valley Biomedical heat inactivation protocol is to bring the temperature of the material to 56 deg C and maintain this for 30 minutes. Some precipitation may be evident, which can be removed, if desired, by filtration. | |
Why blood type AB?
The Human AB+ Serum lacks antibodies against the A and B blood type antigens since it is best to avoid sera containing any antibodies (not just anti-ABO antibodies) that could react with the cultured cells, especially if the cells are destined to be used for therapy.
For example, a woman who has had multiple pregnancies may develop antibodies against the father's MHC antigens, which are "transplantation" antigens on the fathers' cells and on the involved fetus's cells. Significant levels of antibody circulating in the blood from this source are rare. Nevertheless, like the anti-ABO antibodies, it's best to avoid sera containing antibodies for the reason noted above, if the test system is sensitive to them.
